Alpha-, gamma- and beta-proteobacteria detected in legume nodules in Latvia, using full-length 16S rRNA gene sequencing

Rhizobia are soil bacteria that form nodules on legumes and fix atmospheric nitrogen, reducing the need for chemical fertilisers. In most studies rhizobia described as alpha-proteobacteria have been studied extensively several decades. However, in recent years an increasing number of present beta- gamma-proteobacteria strains potential rhizobia-like bacteria. this study we identify isolated from various grown Latvia, using a full-length 16S rRNA gene sequence, used more precision, compared with partial sequence gene. Bacterial 1962–2019, part which unique historical collection. This is first time bacterial Latvian soils identified molecular biology methods. A wide taxonomic diversity was detected – species α-, β-, γ-proteobacteria classes Paenibacillus polymyxa Bacilli class. P. were only collection, while β- obtained newly specimens, uncovering new commercial legume seed inoculum. Soil samples collected, phosphorus levels determined. Several indicate phosphate solubilising properties.